Reconstitution of CF0F1 into liposomes using a new reconstitution procedure

Reconstitution of flippase activity into liposomes.

Phospholipids are asymmetrically distributed in the plasma membrane of eucaryotic cells, the aminophospholipids being mostly on the cytosolic leaflet, whilst the choline containing phospholipids (phosphatidylcholine and sphingomyelin) are predominantly exposed on the outer leaflet [1]. There is strong evidence in support of the role of specific proteins (" flippases ") that are involved in the ...

Reconstitution of Sand Specimens Using a Rainer System

Air pluviation is a method of preparing laboratory models to carry out many geotechnical experiments. In this study, a new air pluviation device has been developed by the researchers. For laboratory modeling, it is important to calibrate the device using air pluviation to produce accurate specimens with desirable conditions and relative densities. A series of laboratory tests were conducted to ...

Functional Reconstitution into Liposomes of Purified Human RhCG Ammonia Channel

BACKGROUND Rh glycoproteins (RhAG, RhBG, RhCG) are members of the Amt/Mep/Rh family which facilitate movement of ammonium across plasma membranes. Changes in ammonium transport activity following expression of Rh glycoproteins have been described in different heterologous systems such as yeasts, oocytes and eukaryotic cell lines. However, in these complex systems, a potential contribution of en...

Purification and reconstitution into liposomes of an integral membrane protein conferring immunity to colicin A.

The immunity protein to colicin A protects producing cells from the action of this pore-forming toxin. It is located into the cytoplasmic membrane. This protein has been 'tagged' with an epitope from the colicin A protein for which a monoclonal antibody is available. The fusion protein (named VL1) has been purified after extraction from the membrane in two steps using a chromatofocusing and an ...

Reconstitution of purified bacterial preprotein translocase in liposomes.